Mebatic Faq’s

FREQUENTLY ASKED QUESTIONS (FAQs)

Differentiate between aerobes and anaerobes

Unlike aerobes who require oxygen for its growth and survival, an anaerobe is any faq'sorganism that does not require oxygen for growth and may even die in its presence.

List problems with detection of anaerobic infections

Practically it is very difficult to detect anaerobic microorganisms & factors that may limit detection of some of these organisms are:

  • The lack of any diagnostic method other than Gram stain and culture
  • Difficult to isolate from infectious sites and are often overlooked. Isolation requires appropriate methods of collection, transportation and cultivation of specimens.
  • Delivery of culture to the laboratory should be prompt, and transport devices should provide an O2-free atmosphere of carbon dioxide, hydrogen, and nitrogen.
  • Ubiquity on mucocutaneous surfaces that hampers obtaining meaningful cultures
  • The presence of mixed flora including other known pathogens.
  • Anaerobes are destroyed when exposed to the atmosphere for as briefly as 10 minutes.
  • The specimen must be protected from oxygen during collection and transport and must be transported to the laboratory immediately.
  • Most of laboratories in India do not routinely test anaerobic organisms.
  • Antibiotic sensitivity testing of anaerobes is exacting, and data may not be available for ≥ 1 wk after initial culture.
  • Only specimens from normally sterile sites should be cultured because commensal contaminants may easily be mistaken for pathogens.
  • Anaerobes are often accompanied by aerobic organisms & difficult to detect.

What is the composition of MEBATIC

Each 100 ml of glass bottle contains

  • Ofloxacin : 200 mg
  • Ornidazole : 500 mg

What is the mechanism of action of MEBATIC

Ofloxacin inhibits the enzyme Bacterial DNA gyrase,which nicks double standard DNA introduces negative super coils and then repeals the nicked ends, Ofloxacin binds to A subunit with high affinity and interfere with it’s strand cutting & repealing function

Ornidazole after entering the cell by diffusion it’s nitro group is reduced by certain redox Proteins operative only in anaerobic microbes to highly reactive nitro radical which exerts cytotoxicity by damaging DNA & other critical molecules.

What is the dose of MEBATIC

One bottle twice daily over a period of 30 min for 3 – 7 days depending upon the type and severity of infection

What is the drip rate of MEBATIC

It has a drip rate of 3 ml/ min

What is the Spectrum of activity

Anaerobes

  • Gram-positive: Clostridium spp., Peptostreptococcus spp.
  • Gram Negative: Bacteroides spp. Prevotella melaninogenica and Prevotella intermedia. Fusobacterium nucleatum,. E.coli, Klebsiella pneumoniae, Proteus mirabilis, Salmonella like
    S. enteriditis , S. typhi, Shigella & Yersinia spp. Serratia spp., Vibrio spp.

Aerobes

  • Gram-positive: Staphylococcus aureus (methicillin-susceptible strains),Streptococcus pneumoniae (penicillin-susceptible strains) Streptococcus pyogenes, Staphylococcus epidermidis (methicillin-susceptible strains)
  • Gram-negative: Gardnerella vaginalis

Protozoa

  • Entamoeba histolytica, Giardia lamblia, Cryptosporidium parvum, Trichomonas vaginalis